The Highest Standard in DNA Sequencing Services
We currently offer the following DNA Sequencing-related services:
Large-scale DNA sequencing (>100 samples)
Small-scale DNA sequencing (<100 samples)
Plasmid DNA preps from glycerol stocks in advance of sequencing
PCR Products Sequencing
Key Features
· Multiple of 3730XL sequencers stand by
· Sequence up to 800bp per reaction, more than 90% successful rate
· Special prices on large projects and contracts
· 24hr to 48 hour turnaround
· Incoming sample QC
· Fluorescent dye-terminator sequencing
· >500bp Q20 read lengths typical
· Universal primers provided at no additional charge (complete list)
· Template preparation and primer synthesis available
FREE SHIPPING
> 100 samples run, we offer FREE SHIPPING
How to Order
1. Complete the sequencing order form online
You may order DNA sequencing online through Bio Basic Inc. website www.biobasic.com by filling the Online Order Form. Options are available for submitting one to hundreds of samples.
2. Download and Email: You may download Excel Sequencing Order Sheet from www.biobasic.com and email to sequencing@biobasic.com and copy orders to order@biobasic.com
3. We offer easy payment options via purchase order number and/or credit card.
4. Send the samples
Phone | 905-474-4493 905-474-4493 |
Fax | 905-474-5794 |
Email | Sequencing@biobasic.com order@biobasic.com |
Web | www.biobasic.com |
Mail | 160 Torbay Road Markham, Ontario, L3R 1G6 |
Samples | Free Pick Up if >100 Samples (Canada/USA only) |
5. Electronic Results sent after 24 hours to 48 hours
CODE | NAME | SIZE | PRICE (USD) |
SEQ-1 | Bacterial/Plasmids /PCR | 1-10 samples | $5.00 ** |
SEQ-10 | Bacterial/Plasmids /PCR | 10-99 | $4.80 ** |
SEQ-100 | Bacterial/Plasmids /PCR | 100-500 | $4.50 ** |
SEQ-500
SEQ-Bulk | Bacterial/Plasmids /PCR
Bacterial/Plasmids /PCR | > 500
Project Based | Inquire **
Inquire ** |
SEQ-OLIGO | Primer synthesis | BASE | $0.20 |
Sample Requirement
We accept plasmid DNA (100ng/ul, 20ul), bacteria, PCR products (crude or purified).
Note: Please specify vector name and insert length and resistance.
PCR (crude): concentration > 30ng/ul, volume >50ul
PCR (purified): concentration > 30ng/ul, volume > 20ul
Note: PCR sample, please indicate the length of insert (bp)
Please indicate the concentration of the primer enclosed with sample.
The following primers are provided free of charge: M13+, M13-, T7, T3, T7 Terminator, SP6, pGEX5’, pGEX3', BGH rev.
Samples are permanently destroyed after two months.
DNA Preparation Prices
Plasmid Purification: $1.00 per well
PCR Clean Up: $0.60 per well
*** Samples are accepted as bacterial colonies, glycerol stocks, pelleted cultures, crude PCR product
Terms and Conditions
Terms
Bio Basic Inc. will send electronically DNA sequencing results to customers. All results will be kept in Bio Basic Inc.’s data base for 6 months. During this period, customer may request sequencing results. After 6 months, DNA sequencing results are automatically destroyed without further notification.
DNA sequencing tests are not considered confirmed without customer’s providing a P.O. number or credit card number. Bio Basic Inc. accepts credit card (Visa or MasterCard), cheque, or wire in payment.
Turnaround
Bio Basic Inc. will make its best effort to send DNA sequencing results within 48 hours. For Larger projects, time will vary depending on the size of the project. We try our best to accommodate special time constraints whenever possible, and projects of more than one hundred reactions may still get 24-48 hours turn around. Mail-in samples need to be received before 12.00 noon time to catch the same day run (results available the next day). If you are using FedEX, priority overnight arrives here each day at about 11:00 am
Cancellations
Buyer may not cancel any order or return any samples without Bio Basic Inc.’s consent. Cancellation and return charges may be charged. Buyer must contact Bio Basic Inc. to obtain a return material authorization number.
Warranty
Bio Basic Inc. guarantees > 500bp Q20 read length and more than 90% successful rate. Bio Basic Inc. does not guarantee any downstream application(s) or usage of the DNA sequencing results. Any claims or dispute must be submitted within 2 weeks after shipment. At the time of shipment, Bio Basic Inc. keeps a data base for unexpected incidents and/or disputes. Bio Basic Inc. reserves the right to refuse handling disputes after period of 3 months.
Prices
Bio Basic Inc. unit prices for DNA sequencing tests apply only to the specific quantity, sample purification and primer synthesis. All prices are subject to correction of errors; Bio Basic Inc. reserves the right to adjust prices on orders during production due to changes in cost of materials, transportation or wages. Failed runs will be charged in full however Bio Basic Inc will repeat failed samples at least once. Any sample that sequences with secondary structures will not be considered a failed reaction and will not be repeated. Any tax, customs, surcharge or duty, howsoever denominated, imposed upon the sale, importation, delivery or use of Products shall be the responsibility of Buyer.
