One of the Largest Professionals in Oligo Synthesis

Features:
 
. Highest Purity at Lowest Price
. Specializing in HAP* (High Affinity Purification) and PAGE Purification
. Worldwide Services
. High Capacity: 5000 primers/day
. One of the Largest and the Most Experienced Suppliers
. Fast Services: Synthesis Completed within 24 or 36 Hours (for HAP* or PAGE)
. All modified base oligos are purified by HPLC (WAVE system)
. Highest QC and Quality Guaranteed
. Wide Range Product Lines
 
High Affinity Purification (HAP) is a patented, novel purification method for custom oligos developed by Bio Basic Inc. DMT-ON-Oligo in the crude oligo mixture is first selectively absorbed on a high affinity resin in HAP column while incomplete oligos pass through. Final oligo is produced by removing the protection group of DMT under mild acidic conditions. HAP method provides two major advantages, high purity superior to De-Salted method (>98%), and low cost comparable to PAGE or HPLC methods. Oligos produced by the HAP method has such high purity that they can be directly used for any downstream experiments such as PCR, DNA Sequencing, Gene Synthesis, and Mutagenesis. At present, the most economic method to produce oligos is the De-Salted method, which however, yields products poor in purity. For example, the purity of 20-mer, 40-mer and 60-mer is approximately 68%, 45% and 30% respectively. This is calculated based on the 4 steps in DNA synthesis: De-DMT, Coupling, Oxidation and Capping. The average yield of each cycle is about 98%. The purity of 20-mer is therefore (0.98)20-1 = 68%; of 40-mer = (0.98)40-1 = 45%; and of 60-mer = (0.98)60-1 = 30%. Most laboratories use De-Salted oligos despite of their inferior purity because higher quality oligos produced by alternative methods such as PAGE, HPLC, and OPC are too costly. HAP presents the perfect alternative for high purity oligos at lowest prices. In fact, price is even lower than those of De-Salted method in some cases.
 
To place an order:
 
. Download Oligo submission form online www.biobasic.com
. E-mail order to: order@biobasic.com
. Contact your local distributor
. Please indicate scales, purification method, 5' end or 3' end modification
. To guarantee quality, we do not carry De-salted oligos.  HAP is a superior purification scale than Desalt.