Protein Expression and Purification service!

Guaranteed 15 mg purified soluble protein for only $2,880!

Introduction
Bio Basic Inc., an industry leader in gene, peptide synthesis, is pleased to launch the new protein expression and purification service. Bio Basic Inc. has the necessary know-how to deliver the best possible results to our valued customers. We have the capacity to take your project directly from gene synthesis to protein expression, refolding and purification. At Bio Basic Inc., we are proud of our ability to optimize protein expression, maximize product yield, and ensure purity to serve every aspect of your research needs.

From Gene to Protein
Starting from de novo gene synthesis, cDNA or from almost anywhere a client asks us to begin; our scientists can deliver high quality purified protein in a timely manner.

About Protein Purification
Recombinant proteins are used throughout the whole spectrum of biomedical research. Once you have large quantity of purified protein, you could conduct many downstream experiments, such as enzyme kinetics, functional and structural studies.

There are two main systems for the expression of recombinant protein - prokaryotic and eukaryotic.

Prokaryotic recombinant protein expression systems (E.Coli) have several advantages, including ease of culture, rapid cell growth and relatively simple purification procedures. However, most post-translational modifications are not added by bacteria, and most proteins become insoluble in inclusion bodies and are very difficult to recover as functional proteins.

Eukaryotic systems for the expression of protein include yeast, baculovirus cells, and mammalian cells. Advantages of these systems include high levels of expression, no inclusion bodies, and intact post-translational modifications.

Things to Consider
Sequence Optimization: In highly expressed genes, a narrow set of codons is used and these codons correspond to the more abundant tRNA species. This minimizes the risk of tRNA depletion during translation. In fact, the codons in a gene may be true bottlenecks, especially in cases where foreign genes are expressed in a host in which the usage of codons in highly expressed genes does not resemble the usage of codons in the species from which the foreign gene originates. In such cases, it has been shown that substitution of rare codons in the introduced gene may increase the yield dramatically. In addition, replacement of rare codons might decrease the chance of misincorporation and protect the protein from premature turnover (1).

Expression System: In order to decide which host is most suitable the amount and the degree of purity of the product as well as its biological integrity and potential toxicity should be considered. For example, bacterial expression systems are not suitable if post-translational modification is required to produce a fully functional recombinant product (2).

Expression Vector: Once the host has been selected, many different vectors are available for consideration. However, as for the selection of a suitable host system, the final choice of vector should take into consideration the specific requirements of the application and will, of course, be influenced by the behaviour of the target protein.

Tag: As for the selection of host and vectors, the decision to use which tag must be made according to the needs of the specific application. The two most commonly used tags are glutathione S-transferase (GST tag) and 6 x histidine residues ((His)₆ tag).

Upon receiving of sequence information, our scientists would analyze the sequence to determine characteristics like molecular weight, pI, solubility, susceptibility to oxidation and hydrophobicity. Every protein is different, there is no standard way for protein expression and purification, and the protocols and strategies must be worked out for each individual protein and with its intended use in mind. Scientists at Bio Basic Inc. use their accumulated extensive working knowledge of purifying recombinant protein to best tailor our service to serve your research needs. At Bio Basic Inc., we offer all four expression systems* to give you the maximum flexibility.

Protein Expression Systems at Bio Basic Inc.

1. E.Coli Expression System
Escherichia coli (E. coli) is one of the most widely used hosts for the production of heterologous proteins. Its genetics are far better characterized than those of any other microorganism....more

2. Baculovirus Expression System
The Baculovirus insect cell expression system is widely used for the production of precisely matured, folded and processed recombinant proteins. In contrast to E. coli, insect cells are able to incorporate post-translational modifications. This unique tool usually yields high amounts of the target protein, making it highly cost effective in comparison to other eukaryotic expression systems.... more

3. Mammalian Expression System
Mammalian cell lines (CHO, HEK 293 etc) are widely used for the production of recombinant glycoproteins (vaccines, enzymes) as well as hormones and immunobiologicals (antibodies, interleukins, lymphokines), as they have the advantage of full posttranslational modifications of the expressed protein .... more

* Yeast expression system is available upon request.

Price and Services Summary

1. E.Coli Expression System

Description	Deliverables	Price
Guaranteed 15 mg purified soluble protein    (Cat# PUR-15MG-ECOLI) Subcloning Expression optimization and verification Expression and purification QC by SDS-PAGE, Western blot* Tag removal, endotoxin removal service available	Guaranteed 15 mg purified soluble protein from E.Coli expression system QC data	From $2,880
Guaranteed 5 mg purified soluble protein     (Cat# PUR-5MG-ECOLI) Subcloning Expression optimization and verification Expression and purification QC by SDS-PAGE, Western blot* Tag removal, endotoxin removal service available	Guaranteed 5 mg purified soluble protein from E.Coli expression system QC data	From $2,110
Traditional Service   (Cat# PUR-TRAD)   Subcloning Expression optimization and verification Expression and purification QC by SDS-PAGE, Western blot* Tag removal, endotoxin removal service available	Purified  protein from E.Coli expression system QC data	Please inquire