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Gene Synthesis FAQs

Below are some frequently asked questions about our Gene Synthesis services. Should you have any questions other than listed below, feel free to contact us at gene@biobasic.com and we will be happy to clear your thoughts.

  1. 1. What are the deliverables?
  2. 2. How would I know the status of my genes?
  3. 3. What is the standard vector for Bio Basic gene synthesis?
  4. 4. Can Bio Basic subclone the gene into the vector of my choice?
  5. 5. What kinds of payments do you accept?
  6. 6. Do you provide protein expression services for codonoptimized genes?
  7. 7. Are your genes sequencing verified?
  8. 8. What are non-standard sequences?
  9. 9. What are the Terms and Conditions for Gene Synthesis?
1

What are the deliverables?

Generally, the deliverables for your synthetic gene of interest will consist of the following:

  • 2-4ug of lyophilized DNA (up to 10ug upon request at no extra cost).

  • Sequence chromatogram tracefiles.

  • Sequencing Results.

  • Final plasmid sequence.

  • Alignment files.

  • Reconstitution, re-plating and bacteria growth instructions.

  • Vector information.

  • Gene report (includes restriction digestion data).

2

How would I know the status of my genes?

You will receive weekly email updates every Friday (EST) outlining the status of each gene you have requested us to complete.

3

What is the standard vector for Bio Basic gene synthesis?

Bio Basic standard cloning vectors include pUC57-Amp, pUC57-Kan, pUC-SP and pBluescript . In general, synthetic genes will be cloned via Blunt of the standard vector, or any cloning sites you desired. Click here for a list and details on our cloning vectors.

4

Can Bio Basic subclone the gene into the vector of my choice?

Yes, Bio Basic can subclone the synthetic gene into the vector of your choice at an affordable additional cost. The vector (commercial vector or non-commercial vector) must be provided to us with a full-length sequence and other requested info.

5

What kinds of payments do you accept?

Bio Basic accepts credit cards (Visa, MasterCard, American Express), cheques and wire transfer. You may send us a PO if you are an academic institution. For certain orders, Bio Basic may require a deposit.

6

Do you provide protein expression services for codonoptimized genes?

Yes! Bio Basic provides protein expression and purification services using bacteria, yeast, baculovirus, and mammalian cell systems. For more information, please visit our Protein Production service page.

7

Are your genes sequencing verified?

Yes. The sequences of genes synthesized by us are confirmed by DNA sequencing, and 100% guaranteed.

8

What are non-standard sequences?

Generally, it is more difficult to synthesize sequences that have:

  1. i) GC content > 70% or < 40% in regions of at least 30 base pairs or the whole sequence (generally difficult to synthesize sequences that have either too high or low GC content; standard sequences typically have a GC content of 50%).
  2. ii) Long sequence repeats (>12 bp) that exist in a high frequency (interval between repeats < 100bp), e.g. internal repeats, direct repeats or inverted repeats.
  3. iii) Homopolymers longer than 15 bp.
  4. iv) Sequences that have a high similarity with toxic genes.


9

What are the Terms and Conditions for Gene Synthesis?

Please click here for the terms and conditions for our gene synthesis related services.

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