Below are some frequently asked questions about our Gene Synthesis services. Should you have any questions other than listed below, feel free to contact us at email@example.com and we will be happy to clear your thoughts.
Generally, the deliverables for your synthetic gene of interest will consist of the following:
2-4ug of lyophilized DNA (up to 10ug upon request at no extra cost).
Sequence chromatogram tracefiles.
Final plasmid sequence.
Reconstitution, re-plating and bacteria growth instructions.
Gene report (includes restriction digestion data).
You will receive weekly email updates every Friday (EST) outlining the status of each gene you have requested us to complete.
Bio Basic standard cloning vectors include pUC57-Amp, pUC57-Kan, pUC-SP and pBluescript . In general, synthetic genes will be cloned via Blunt of the standard vector, or any cloning sites you desired. Click here for a list and details on our cloning vectors.
Yes, Bio Basic can subclone the synthetic gene into the vector of your choice at an affordable additional cost. The vector (commercial vector or non-commercial vector) must be provided to us with a full-length sequence and other requested info.
Bio Basic accepts credit cards (Visa, MasterCard, American Express), cheques and wire transfer. You may send us a PO if you are an academic institution. For certain orders, Bio Basic may require a deposit.
Yes! Bio Basic provides protein expression and purification services using bacteria, yeast, baculovirus, and mammalian cell systems. For more information, please visit our Protein Production service page.
Yes. The sequences of genes synthesized by us are confirmed by DNA sequencing, and 100% guaranteed.
Generally, it is more difficult to synthesize sequences that have:
Please click here for the terms and conditions for our gene synthesis related services.