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Our Gene Synthesis

Our Gene Vectors

Below is a listing of vectors available in-house. Please inquire about vectors other than listed below, as we may or may not carry them in-house and would need to be shipped to us for subcloning projects. Please inquire at gene@biobasic.com and we will be happy to assist you.

1

pUC57-Amp

Sequence map

"Cloning vector with an ampicillin resistance marker, suitable for generating ExoIII deletions." The pUC57's MCS contain 6 restriction sites with 3’-ends that are resistant to ExoIII.
Source: www.snapgene.com


2

pUC57-Kan

Sequence map

"Cloning vector with a kanamycin resistance marker, suitable for generating ExoIII deletions." Source: www.snapgene.com

3
4

pBluescript II SK+

Sequence map

"Standard cloning vector (phagemid excised from lambda ZAPII). The f1 (+) orientation allows rescue of sense strand ssDNA."
Source: www.snapgene.com

5

pET-21A

Sequence map

"Bacterial vector for inducible expression of N-terminally T7-tagged proteins."
Source: www.snapgene.com

6

pET-28B

Sequence map

"Bacterial vector for expression of N-terminally 6xHis-tagged proteins with a thrombin site."
Source: www.snapgene.com

7
8

pBluescript II KS+

Sequence map
Source: www.snapgene.com

9

pBluescript II SK-

Sequence map
Source: www.snapgene.com

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11
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