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Our Gene Synthesis

Our Gene Vectors

Below is a listing of vectors available in-house. Please inquire about vectors other than listed below, as we may or may not carry them in-house and would need to be shipped to us for subcloning projects. Please inquire at gene@biobasic.com and we will be happy to assist you.

Complementary Vectors

pUC57 pBR322 pBluescript II KS+
pUC57-Kan pUCm-T pBluescript II SK+
pUC57m (No NdeI) pUC19 pBluescript II SK-

More Vectors

Subcloning fees apply to the following vectors
pUC18 pTT5 pCold II
pET-3a pET-9a pET-11a
pET-15b pUC19 pET-19b
pET-20b(+) pET-21a(+) pET-21b(+)
pET-22b(+) pET-24a(+) pET-24b(+)
pET-28a(+) pET-28b(+) pET-29b(+)
pET-30a(+) pET-32a(+) pET-32b(+)
pET-41a(+) pET-45b(+) pET-47b(+)
pET-48b(+) pET-49b(+) pET-50b(+)
pEGFP-C1 pEGFP-C3 pcDNA3.1 (+)
pGEX-4T-1 pGEX-6P-1 pGL3-Basic
pGL3-promoter pQE-30 pQE-60
pACYCDuet-1 pCDFDuet-1 pETDuet-1
pGL4.17[luc2/ Neo] pGL4.19[luc2CP Neo] pIRES2-EGFP
pmirGLO
1

pUC57-Amp

Sequence map

"Cloning vector with an ampicillin resistance marker, suitable for generating ExoIII deletions." The pUC57's MCS contain 6 restriction sites with 3’-ends that are resistant to ExoIII.
Source: www.snapgene.com


2

pUC57-Kan

Sequence map

"Cloning vector with a kanamycin resistance marker, suitable for generating ExoIII deletions." Source: www.snapgene.com

3
4

pBluescript II SK+

Sequence map

"Standard cloning vector (phagemid excised from lambda ZAPII). The f1 (+) orientation allows rescue of sense strand ssDNA."
Source: www.snapgene.com

5

pET-21A

Sequence map

"Bacterial vector for inducible expression of N-terminally T7-tagged proteins."
Source: www.snapgene.com

6

pET-28B

Sequence map

"Bacterial vector for expression of N-terminally 6xHis-tagged proteins with a thrombin site."
Source: www.snapgene.com

7
8

pBluescript II KS+

Sequence map
Source: www.snapgene.com

9

pBluescript II SK-

Sequence map
Source: www.snapgene.com

10
11
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