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Bio Basic is one of the largest professional and affordable gene manufacturers in the world. For over 15 years, Bio Basic as a silent OEM has synthesized millions of base pairs of genes for researchers worldwide.
From large scale projects to small custom projects, we can assist you in selecting the service which would offer you the greatest benefit.
Our reputation as gene synthesis specialists is consistently emphasized as our competitors fail to deliver large (5kb to 25kb+) and complex genes, whereas we have an over 95% completion rate on even the most challenging of sequences. Any company which relies purely on silicon well-based platforms is limited to the construction of small and simple genes, thereby limiting their ability to work on a significant portion (up to 50%) of gene projects.
We are able to accomplish these feats due to over a decade of experience, in which on top of using the chip technology, we have developed and utilized proprietary enzymes and reagents in our production process, while following gold-standard synthesis practices.
Go to the Gene Order Portal to submit your gene order, or get an automated Quote
PORTAL ADVANTAGES:
Instant Quotes: Get your quotes instantly
Bulk Gene Imports: Easily import over 100 genes at once
Invoice History: Easy access to invoices
Order History: Easy access to gene order history
Gene Data files: Easy access to your gene data files
Consolidated data: All your gene info and data in one place
Click here for instructions on how to use the portal
Bio Basic offers Gene Services for projects related to the COVID-19 and the Omicron variant. Please mention "Coronavirus", "COVID-19", "Omicron", "booster" or "vaccine" in the comments section upon checkout.
We offer the highest quality gene synthesis services at the most affordable rates in the industry. We can match or beat any competitor's price (some conditions apply).
We are experts in long and complex genes, with the highest success rates in the industry. In addition, each gene synthesized by us is verified by DNA sequencing.
We understand that your projects can be highly confidential, and we thoroughly agree to not release any information to a third party.
We have a simple Gene Project Tracker portal where you can use your designated BBI ID(s) to track your project progress.
Zhao, Evan M., Yanfei Zhang, Justin Mehl, Helen Park, Makoto A. Lalwani, Jared E. Toettcher, and José L. Avalos. "Optogenetic regulation of engineered cellular metabolism for microbial chemical production." Nature 555, no. 7698 (2018): 683.
Diver, Melinda M., Leanne Pedi, Akiko Koide, Shohei Koide, and Stephen B. Long. "Atomic structure of the eukaryotic intramembrane RAS methyltransferase ICMT." Nature 553, no. 7689 (2018): 526.
Daou, Salima, Haithem Barbour, Oumaima Ahmed, Louis Masclef, Caroline Baril, Nadine Sen Nkwe, Daméhan Tchelougou et al. "Monoubiquitination of ASXLs controls the deubiquitinase activity of the tumor suppressor BAP1." Nature communications 9, no. 1 (2018): 4385.
Hosford, Christopher J., and Joshua S. Chappie. "The crystal structure of the Helicobacter pylori LlaJI. R1 N-terminal domain provides a model for site-specific DNA binding." Journal of Biological Chemistry (2018): jbc-RA118.
Xu, Jie, Guennadi Kozlov, Peter S. McPherson, and Kalle Gehring. "A PH-like domain of the Rab12 guanine nucleotide exchange factor DENND3 binds actin and is required for autophagy." Journal of Biological Chemistry (2018): jbc-RA117.
Qi, Xiaodong, Fei Zhang, Zhaoming Su, Shuoxing Jiang, Dongran Han, Baoquan Ding, Yan Liu, Wah Chiu, Peng Yin, and Hao Yan. "Programming molecular topologies from single-stranded nucleic acids." Nature communications 9, no. 1 (2018): 4579.
Du, Ting, Nakita Buenbrazo, Laura Kell, Sadia Rahmani, Lyann Sim, Stephen G. Withers, Shawn DeFrees, and Warren Wakarchuk. "A Bacterial Expression Platform for Production of Therapeutic Proteins Containing Human-like O-Linked Glycans." Cell Chemical Biology (2018).
More Citations (via Google Scholar).
Multiple, long primers (~50-60 bases in length) with similar melting temperature (Tm) are chemically synthesized as fragments based on the desired final gene sequence. Primers are designed to assemble with each other through overlapping sequences.
Using PCR, the primer fragments are assembled into blocks of up to 1kb of double stranded DNA.
These blocks are then assembled and amplified once again using PCR to create one large double stranded DNA construct.
All genes are verified by DNA sequencing and only those with the correct sequences are selected. Here, Sanger Sequencing is used due to its long read length short run time.
In the event errors are identified, we perform error correction work to correct mutated bases. This ensures that the gene synthesized conforms to the specifications planned in the beginning of the project.
The synthesized DNA is inserted into a specific vector. The vector is then isolated and amplified. Unless otherwise specified, the default vector used is the pUC57-Amp vector.Click here to view our vector list.
Colonies are grown and appropriate ones are then selected containing the synthetic gene of interest.
All genes are verified by DNA sequencing and only those with the correct sequences are selected. Here, Sanger Sequencing is used due to its long read length short run time
Further QC measures are applied and the gene is lyophilized into a microcentrifuge tube. The gene is now ready for downstream applications.
Through the introduction of next generation gene synthesis method - CHIP technology, pricing for gene synthesis is expected to be significantly reduced over the coming years. Therefore, it will be a great asset to have a partner who is capable of adjusting to this changing market.
Bio Basic strives to stay competitive in the marketplace through strategic investment in new technologies such as the parallel on-chip gene synthesis technology.
Having Bio Basic as your partner will enable you to continue to meet and exceed your expectations and needs, without the growing pressure and complications that may arise from switching partners in this ever-changing market.
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