Home / Diagnostic Reagents/Enzymes/Probes / Multiplex

Introduction

The 2x Multiplex PCR Master Mix is a ready-to-use solution containing dNTPs, MgCl₂, multiplex PCR Buffer, and PCR additives designed to improve amplification efficiency and reduce primer-dimer formation. Users need to add template DNA, a primer mix, the provided HS Taq DNA Polymerase, and water to set up the PCR reaction.

Our proprietary HS Taq DNA Polymerase is a hot-start enzyme, inactive at ambient temperatures, preventing nonspecific binding and primer-dimer formation during setup. It is activated by a 4-minute incubation at 95°C. This allows for convenient reaction setup at room temperature.

The 2x Multiplex PCR Master Mix already provides a final MgCl₂ concentration of 4mM in the reaction, which is optimal for most multiplex PCRs. MgCl₂ optimization by titration is usually not required as primer annealing is only marginally influenced.

Contents

Kit Components