From Design to Discovery: The Power of Gene Synthesis in Your Research

Gene synthesis, also popular as artificial DNA synthesis, is an emerging process that empowers scientists and researchers to generate specific DNA sequences. This groundbreaking mechanism has transformed the domain of research and biology, opening doors to countless applications in machine, biotechnology, and basic research.

Understanding Gene Synthesis

A gene is the basic component of heredity, carrying the instructions for building protein components. Genes are composed of DNA (deoxyribonucleic acid), a double-stranded molecule that encrypts genetic information. A comprehensive gene synthesis service allows scientists to construct artificial DNA sequences based on specific genetic codes. The process effectively replicates the natural process of DNA replication but in a controlled laboratory setting.

The Custom Gene Synthesis Process: A Step-by-Step Breakdown

Gene synthesis is a meticulous process that involves several key steps. Let’s take a glance at the following:

Design:

The first step involves meticulous design in accordance with the DNA sequence. Professional scientists utilize specialized tools and software to craft the sequence, taking into account essential factors like compatibility with the intended application, codon optimization, and the presence of regulatory elements.

Oligodeoxynucleotide (Oligo) Synthesis:

Once the DNA sequence is designed, the process moves on to oligo synthesis. These oligos are short, single-stranded DNA fragments that are chemically synthesized in automated machines. These oligos act as the building blocks for the final DNA molecule.

Fragment Assembly:

The synthesized oligos are later assembled meticulously into a complete DNA molecule. This assembly can be achieved using various processes, such as enzymatic ligation or polymerase chain reaction (PCR).

Purification And Verification:

The pre-arranged DNA fragments now undergo a rigorous purification process to eliminate any impurities or contaminants. Finally, the synthesized DNA is sequenced to demonstrate that it perfectly matches the designed sequence.

Different Applications of Gene Synthesis:

Gene synthesis underpins a wide range of groundbreaking applications, including:

Gene Therapy:

Gene synthesis plays a vital role in gene therapy, a process that aims to treat genetic diseases by bringing up functional copies of genes into patients’ cells.

Synthetic Biology:

Gene synthesis is the cornerstone of synthetic biology, a domain that centers around designing and engineering biological systems. Scientists can generate novel organisms with entirely new functionalities or tailor existing organisms for specific purposes.

Protein Production:

Synthetic genes can be established in host organisms for the production of specific proteins. This application is particularly applicable in the production of therapeutic proteins, and enzymes.

Bio Basic: Your Trusted Partner in Finding the Best Custom Gene Synthesis Service

Bio Basic is an outstanding provider of top-quality gene synthesis services. We leverage cutting-edge technology and stringent quality control procedures to ensure the accuracy and reliability of synthesized genes. Our team of experienced scientists possesses in-depth knowledge of gene synthesis and is dedicated to providing exceptional customer support. Whether you require the synthesis of a simple gene or a complex construct, we are your trusted partner. We offer competitive rates, rapid turnaround times, and a commitment to exceeding your expectations.

In conclusion, gene synthesis is a powerful tool that is revolutionizing the field of biology. With its diverse applications, gene synthesis holds immense potential for advancements in medicine, biotechnology, and our overall understanding of life itself. If you are considering incorporating gene synthesis into your research endeavors, Bio Basic stands ready to be your partner in scientific exploration.

Leveraging our expertise in gene synthesis can accelerate your research and development efforts. Contact us today to discuss your specific requirements and explore how we can empower your scientific breakthroughs.

Top Things You Need To Know About Artificial Gene Synthesis

Artificial gene synthesis has become an essential tool in biotechnology's rapidly advancing field, ushering in an era of scientific innovation and medical breakthroughs. Transcending traditional boundaries, artificial gene synthesis enables de novo construction of genes from nucleotides obviating template DNA requirements - thus speeding research progress while opening up limitless possibilities in genetic engineering and synthetic biology.

The Essence of Artificial Gene Synthesis:

Artificial gene synthesis involves two key steps. First, solid-phase DNA synthesis - commonly referred to as DNA printing - generates short oligonucleotide fragments with typically 200 base pairs or less in length. Next, these fragments are assembled using various DNA assembly methods into whole new DNA molecules without recourse to natural sequence templates, allowing scientists to explore all their genetic possibilities.

How does Artificial Gene Synthesis Work?

Artificial gene synthesis involves the construction and assembly of DNA sequences from nucleotides de novo, without the need for a template DNA. Let's have a look -

1. Sequence Optimization and Oligo Design: The desired DNA sequence is optimized for the desired application, and oligonucleotides (short DNA fragments) are designed to cover the entire sequence.
2. Oligo Synthesis: The oligonucleotides are synthesized using automated chemical synthesis methods.
3. Gene Assembly: The oligonucleotides are assembled into larger DNA fragments using various DNA assembly methods, such as ligation or polymerase cycling assembly (PCA).
4. Sequence Verification and Error Correction: The assembled DNA fragments are verified for sequence accuracy using techniques like Sanger sequencing or next-generation sequencing.
5. Preparing synthetic DNA for downstream applications: The synthetic DNA is prepared for use in various applications, such as cloning into expression vectors or assembly into larger constructs.

What are the Applications of Artificial Gene Synthesis?

Artificial gene­ synthesis has a significant impact, influencing all realms of life­ sciences and biotechnology. From crafting the­rapeutic antibodies and cultivating resilie­nt crops to cloning research, artificial gene synthesis se­rves a pivotal function in discovering innovative re­medies to worldwide issue­s. Additionally, this method also plays a pivotal role in protein e­ngineering, neuroscie­nce study, cancer biology investigation, vaccine­ formulation, protein enginee­ring advancement as well as protein e­ngineering advanceme­nt ventures - demonstrating its fle­xibility and transformative power.

What are the Advantages of Artificial Genen Synthesis?

Artificial gene synthesis offers numerous advantages, marking a transformative change in genetic research and biotechnology. It offers unrivaled time and cost efficiencies, enhanced DNA performance via codon optimization, elimination of traditional cloning constraints and guarantees of sequencing accuracy as well as the creation of large complex genes - benefits which underscore its critical role in driving diagnostic and therapeutic innovation forward.

What is the Role of Artificial Gene Synthesis?

Artificial gene synthesis holds significant implications for biotechnology and beyond and represents a giant leap forward in our ability to understand life itself. Artificial gene synthesis' journey, from concept to application, is not only an example of human ingenuity but also offers hope for medicine, agriculture and environmental sustainability in the coming years. By adopting this technology we open up its potential to address some of humanity's most urgent issues while ushering in a new era of innovation and discovery.

Bio Basic: Pioneer of Artificial Gene Synthesis:

Bio Basic stands out as a leader in artificial gene synthesis services, offering unparalleled expertise and affordability. Boasting over 15 years of experience in successfully synthesizing complex gene sequences, we represent innovation at its finest in this field. Our skill in handling large and intricate gene projects, along with our custom gene synthesis services and commitment to customer confidentiality and service, sets us apart from other gene synthesis service providers.

Why Choose Bio Basic?

At Bio Basic, our expertise in handling long and complex genes, and affordable gene synthesis costs make us your go-to choice for gene synthesis projects of any complexity. We meet high-quality expectations and our services are also tailored specifically to the unique requirements of researchers and industries alike.

Conclusion:

Artificial gene synthesis plays a central role in shaping the future of biotechnology and natural sciences, offering us a glimpse of a world limited only by our imagination. As we uncover more and more of life at its molecular core, artificial gene synthesis gives us hope for an endlessly promising future where there are no boundaries to human creativity!

Significance of Custom Antibody Production to Research Applications

In biology and medicine research and development has a long history with antibody reproducibility. In fact, biology-based studies show the major contribution of using antibodies in the basic understanding of the subject. However, these antibodies are also a significant factor in poor reproducibility according to different research. In today's blog, we will take an insight into those studies reflecting the importance of antibody manufacturing in biology research and medicinal development. Keep reading.

Why Is Antibody Reproducibility Important in Research and Development?

Of multiple biology-based studies and research, one study by the Human Protein Atlas shows how antibody generation significantly impacts research applications and where its importance lies. This Swedish consortium manufactures around 9,000 antibodies internally, directing them against human genome proteins to evaluate their efficacy through bioinformatics and experimental data. In the outcome, it’s found that less than 50% of the protein distribution in the preserved tissue slices is active.

In another study, researchers assessed 246 antibodies mostly used in epigenetics fields, where the outcomes reveal that 25% of those reagents failed the test due to displaying a high specificity for wrong molecular targets. That’s why antibody quality issues have become a challenge for researchers.

However, the experts keep taking numerous initiatives to facilitate data availability on antibody quality to improve validation standards as reagents. And, at a point of investigation, they have found out the reasons behind the poor performance of antibodies, whether buying from any commercial sources or generated in-house in research applications. In most cases, this performance failure can occur due to shortcuts, technical oversights or incomplete characterization.

Moreover, custom antibody production is complicated, and each phase can have a significant impact depending on various factors during the creation process. Yet, you need to take careful considerations during every step of the research applications as reagents. It will help you identify the difference between poor-quality antibodies and exceptional ones. For example:

• Exception antibodies come with high specificity and avidity, while poor-quality antibodies contain no or low avidity and specificity.
• Poor-quality antibodies mostly contaminate mycoplasma and viruses with low expression levels. However, with exceptional antibodies, researchers can take advantage of its intended biological function and correct Fc domains. Also, the latter offers proper stability and glycosylation with other desired traits.

When using poor-quality antibodies as a reagent in research applications, it requires characteristic re-optimization from scratch. However, the process can be highly expensive and cause delays in new antibody manufacturing, where the timeline depends on the type of antibody used for your research application, like monoclonal antibody production or polyclonal antibody production.

Being a researcher, the best thing you can do here is to make an upfront investment for the optimization process, ensuring antibody generation meets your needs for the long term. It may increase the initial investment amount and pre-production timeline. But you can save or reduce costs on the scientific and financial consequences of using poor-quality antibodies in the research. So, rely on high-quality antibodies, whether generated in-house or commercially, outsourcing to obtain reproducible and consistent data.

In Conclusion

From the selection to antigen preparation, every step requires careful planning when aiming to develop exceptional antibodies and up-front investment, which increases the chances of high-quality antibody generation. However, if you are looking to save your time and effort, rely on us, Bio Basic, for commercially sourcing antibodies, whether it be chimeric monoclonal antibodies or polyclonal ones.

Just decide which one is the best fit for your application and ask for the results you desire. Our antibody synthesis packages provide the utmost freedom to researchers for antibody synthesis, from cDNA to protein to peptide, and ensure successful basic research applications in the projects of your choice. We have a qualified team of scientists to help you find the best package considering your research goals while informing you about the outcomes throughout the process.

Request a quote now or download our order form and email that to our antibody department at antibody@biobasic.com today!

The Bright Future of Gene Synthesis in the Coming Years

Gene synthesis is the process of artificially constructing genetic material and it has shown rapid growth recently.

As per a market research report, the gene synthesis market was valued at US$ 643.2 million in 2022. And it is expected to grow at an impressive 18.6% CAGR between 2023-2029.

The question here is - what does the future look like for gene synthesis?

Because of the high demand for personalised medicine, gene synthesis has found widespread applications in various areas like genetic engineering, biopharmaceuticals, vaccines, and more.

The falling costs of gene synthesis and sequencing over the last decade have also enabled many startups to enter this market. Investments are pouring in given the large addressable market size.

North America is leading in the adoption of gene synthesis technologies. Progress in fields like CRISPR gene editing and DNA data storage will further fuel the utilisation of artificial gene synthesis. The Asia Pacific market is expected to register the fastest growth in the coming years. As precision medicine and targeted drug development expand, pharmaceutical players will depend more on custom gene synthesis for biologics, DNA-based therapies or even antibody discovery.

However, there are ethical concerns regarding the misuse of artificially constructed viruses and pathogens. Strict biosafety regulations have been introduced for gene synthesis companies. Most firms have rigorous screening procedures in place to prevent hazardous sequences from being manufactured. And, not to forget, improper disposal of genetic waste is another worry. Proper oversight by regulatory authorities may enable further responsible acceleration of gene synthesis technologies.

So, in a nutshell, different factors like enhanced applications, declining costs, supportive regulations and growing investments are responsible for the bright picture for the future of producing synthetic DNA.

And, another fascinating fact is, custom gene synthesis can make drug discovery and development faster, more versatile and economical. With prudent safeguards in place, the promise this field holds for revolutionising healthcare, biofuels, agriculture as well as materials science.

Gene Synthesis Services at Bio Basic

Bio Basic is one of the largest professional and affordable gene synthesis companies globally. For over 15 years, we have silently synthesized millions of base pairs of genes for researchers worldwide. Bio Basic offers services for projects of any scale related to COVID-19, coronavirus variants, vaccines etc.

You can count on us for the affordability of gene synthesis services, expertise in complex long genes, confidentiality guarantee and progress reports for the project. We use advanced gene synthesis techniques beyond silicon-chip technology to reliably deliver large and tricky genes.

Choosing Bio Basic as a gene synthesis partner can help you reap significant benefits for research teams over the coming years.

A Deep Dive into Fetal Bovine Serum, The Lifeblood of Cell Culture

FBS or Fetal Bovine Serum is also known as Fetal Calf Serum. It is a typical component of animal cell culture media broadly used in both industrial research and academic biology sectors as a supplement in cell culture applications for basal growth medium. Fetal bovine serum is usually harvested from pregnant cows' bovine fetuses during slaughter. It is the amber-coloured blood fraction left after the natural clot formation of blood. After that, the centrifugation process is applied for further refining. It removes the serum's remaining blood cells, low-solubility proteins, and coagulation fibrinogens. The final product is a rich source of proteins used as a critical growth factor supporting cell growth in culture.

Today, we will provide a comprehensive overview of Fetal Bovine Serum (FBS) and its uses. Finally, we will also reveal the name of the leading life science research-support company offering USDA-approved US-origin FBS. Let's begin.

Fetal Bovine Serum Overview

FBS is an extensively used serum supplement for the in vitro cell culture of eukaryotic cells. It has many growth factors and also contains a lower level of antibodies. These factors pave the way for versatility in different cell culture applications. Bovine Serum Albumin (BSA), a globular protein, is the major component of fetal bovine serum. FBS also contains many other rich protein varieties that help maintain cultured cells in a medium where they can survive, develop, and divide.

Since FBS is a biological product, its exact composition varies from batch to batch. It causes problems in research as reproducing the result becomes difficult. In fact, in a report published in Nature, Baker said that around 80% of biological researchers cannot repeat the results of others' experiments. 60% of biologists can't even repeat their own experimental outcomes.

To navigate this problem, scientists have developed a serum-free chemically defined medium known as CDM. It is typically made of synthetic ingredients with recognized compositions. As a result, they are more consistent than FBS regarding reducing variability in research results. However, even CDM has some limitations as it lacks many nutrients cells require to grow. Some cell lines still need FBS for optimal growth.

Its Uses

We have already discussed the primary use of fetal bovine serum. It is used as a growth supplement for in vitro cell culture and aids in:

1. Providing nutrients, transport proteins, trace elements, adherence, etc.
2. Supplying hormones for cell proliferation and growth
3. Triggering cell differentiation
4. Creating an appropriate environment for cell growth with detoxifying and stabilizing factors

Academic researchers and scientists from the biotechnological and pharmaceutical sectors also rely on the treasured properties of FBS for many specific applications. The list includes but not limited to:

1. Cloning
2. Biopharmaceuticals
3. Cryopreservation
4. Production of synthetic protein
5. Stem-cell research
6. Animal diagnostics
7. Production of viral vector and vaccine
8. Immunotherapy
9. In vitro fertilization
10. Cell and gene therapy

Buy USDA-Approved US-Origin FBS

Bio Basic, the leading Canadian life science research-support company, offers USDA-approved US-origin fetal bovine serum in two categories. Kindly note that this product is currently only available in Canada. Contact us now to learn more about each option and pricing.

Want to Save Your Research Time and Effort? Outsource Your Cloning and DNA Fragment Needs to Us

Gene synthesis, a common practice of synthetic biology, includes a variety of methods to construct and assemble genes from nucleotides de novo. This process is also known as artificial gene synthesis. Contrasting DNA synthesis in living cells, it does not need template DNA. It allows any DNA sequence to be synthesized in the lab.

The synthesis process contains two key steps. The first of which is called solid-phase DNA synthesis. It is sometimes also known as DNA printing, producing oligonucleotide fragments (typically under 200 base pairs). The next step includes connecting the oligonucleotide fragments by capitalizing on different DNA assembly methods. Since artificial gene synthesis does not require template DNA, making an utterly synthetic DNA molecule is theoretically possible with zero limitations on the nucleotide size or sequence.

Gene synthesis is a highly effective and handy solution for scientists and researchers who want to avoid in-house cloning steps and jump directly to practical studies with fully sequence-verified clonal DNA. Here comes another important question – where should researchers and scientists look for custom gene synthesis services? The rest of this post aims to answer that.

Bio Basic, the leading gene manufacturer, is helping researchers to meet their molecular biology needs without compromising on quality. We have 15+ years of experience in this field. So far, we have synthesized millions of base pairs of genes as a silent OEM for biologists and molecular scientists across the globe. We can also do the same for you. From small custom projects to large-scale operations, you can opt for Bio Basic, irrespective of your requirements.

Our gene synthesis cost is highly economical. However, we don’t compromise with quality in order to make our service affordable. In fact, our reputation as gene synthesis experts is only getting increasingly emphasized as other players from this field have failed to deliver large and complex genes. On the other hand, we have more than a 95% completion rate even when constructing and assembling complex gene sequences.

Most gene synthesis service providers rely on silicon well-based platforms. It limits the construction of genes to small and simple ones. Therefore, they fail to assist in significant parts of challenging gene projects. However, our years of experience in this field helped us to crack this mystery. Even though we use inexpensive technology, our team has successfully developed proprietary enzymes and reagents and figured out the best way to utilize them for production without following substandard synthesis practices.

Along with gene synthesis cloning, we also offer several other services, including oligo-synthesis, DNA sequencing, protein production, antibody production, etc. Get in touch with us to learn more about Bio Basic and our comprehensive solutions. Let our team construct custom DNA with 100% sequence accuracy and help you meet your specific research needs.

Visit us at MEDICA 2023.

website.

Impacts of Automation on DNA Synthesis: A Brief Guide Explained

Recent studies show that the field of synthetic biology has achieved incredible progress with the advancement of technology, for instance, the generation of synthetic RNA and DNA synthesis. All thanks to Automation! The implementation of the automation process in high-throughput DNA synthesis services has made ambitious research projects, like DNA sequencing and drug discoveries, easier than ever!

If you wonder whether automation has any impact in terms of DND synthesis, today’s blog is for you. Let’s get started.

How Is the Automation Process Improving DNA Synthesis Services?

Synthetic biology involves three-part cycles, including design, build, and test, enabling the genome to recognize key molecules and their functions, while driving the modern innovations, whether it be next-generation cell therapies or therapeutic antibody developments. Now, the progress depends on the tools, making the workflows more efficient, where automation comes into play!

Automation allows synthetic biology to keep pace with different technologies, such as RNA and DNA synthesis, rapid DNA sequencing, biophysical screening, and much more. Moreover, it accelerates the testing and design of the sequences, ensuring to meet the demand in biotechnology and pharmaceutical research. Otherwise, the turnaround times can be too long for complex projects, in particular.

That’s why different pharmaceutical and biotechnology researchers are eliminating the implementation of synthetic biology bottlenecks in research, especially DNA synthesis and DNA sequencing services, and embracing automated synthetic biology platforms to welcome the new age of discovery. For example:

Antibody discovery

According to recent studies, antibody-based therapeutic generations in the past identified simple targets, whereas new drug candidates nowadays target more complex ones, such as ion channels and G protein-coupled receptors. Any of such discoveries start with DNA sequencing and end with screening, which are proven to be highly efficient procedures. With traditional DNA synthesis, it may take months due to complex antibody sequencing, whether new drug candidates are synthesized and cloned every time.

But, when there are challenging timelines, automated benchtops in DNA synthesis can save your day by supporting high-throughputs seamlessly during the workflows. Also, it helps researchers determine proprietary vector details to remain safe during the discovery process. Yet, they have adopted automated benchtop systems in high-throughput DNA synthesis processes to drive the success rates of antibody discovery. It takes just a few days to build, clone, and amplify new drug candidates.

Immunotherapy discovery

The application of synthetic biology has also been seen in immunotherapy treatments to streamline the process, such as optimizing and screening chimeric antigen receptors (CARs) and T-cell receptors (TCRs). However, the overall workflows used in CAR T-cell therapies can be slowed down by traditional DNA synthesis services as they take a long turnaround time. Implementing automated high-throughput benchtops will accelerate the discovery of TCR and CAR workflows for researchers.

Technical limitations

In terms of drug discovery and development processes during biotechnology and pharmaceutical research, researchers rely on synthetic biology these days. But, since the sequences are complex, lead times for traditional DNA synthesis become more challenging, especially in the case of high-throughput discovery research. Yet, the workflows get slowed down and delayed, which is untenable when it comes to bringing new treatments to the market quickly.

That’s why researchers turn to automated synthetic biology platforms for DNA synthesis services to ensure dozens of production overnight if required. Moreover, it ensures error-correction capabilities, making it easier for researchers to generate highly accurate DNA products

In Conclusion

Rather than in-house, outsourcing DNA synthesis service mitigates costs and cuts the chances of inconsistencies in accuracy and delays during complex workflows with the use of automation. And, if you are looking for a reliable DNA Sequencing provider, count on us. At Bio Basic, we offer efficient and fast DNA sequencing services using automated benchtops and DNA extraction kits at affordable charges to lower costs and cut turnaround times.

Request a quotation now or place an order via email at sequencing@biobasic.com today!

Importance of Oligonucleotides in DNA and RNA Expression Research

Over the last few decades, molecular biology has revolutionized a lot. And, the most common and critical component researchers have constantly used for DNA and RNA expression research is oligonucleotide. For both RNA and DNA, it's the monomeric element. According to a recent study, biomedicine and biological research wouldn't be at the stage where they are now. In fact, DNA and RNA oligonucleotide synthesis have been proven to be the key to different molecular biology processes, from DNA sequencing to PCR to plasmid construction, genomic manipulation and labeled probes.

Do you wonder why? Keep reading!

Why Is Oligonucleotide a Key Component for DNA and RNA Expression Research?

If it's your first time in DNA or RNA expression research, let's start with what oligonucleotide is.

Well, oligonucleotides are comparatively short single-stranded sequences of nucleotides. However, although the typical oligos for research should be from 5 to 50 bp, these oligonucleotides can be up to 100 bp, allowing a small percentage of DNAs to research. In fact, it's possible to study RNAs by designing oligos.

Now, let's talk about why they are important for biological research, like DNA and RNA expression.

Sequence specificity and complementarity are the prime oligo properties, allowing researchers to identify chemical recognition among nucleotide bases to align specific pairs with each other through hydrogen bonds to form double-stranded molecules. For RNA, it's adenine (A), guanine (G), uracil (U) and cytosine (C), and for DNA, the set remains the same except for thymine (T), replacing uracil (U).

Oligonucleotides used in biological research come the following:

Oligo primer use in PCR

PCR (polymerase chain reaction) is a process to amplify several copies of a specific DNA sequence rapidly from one sample. DNA polymerase needs forward and backward primers to amplify the target DNA to create double-stranded DNA components. Oligonucleotide primers are designed to align with targeted complementary sequences. Make sure you consider annealing and melting temperature!

DNA sequencing

The use of oligonucleotide primers is not limited to just PCR but plays a great role in DNA sequencing. It has the same requirements as the previous one, such as sequencing specificity, annealing and melting temperatures, and hybridization region. However, the set of parameters is different here due to the various conditions of the polymerase reaction.

Plasmid construction

Biological researchers these days use Oligonucleotides for constructing plasmids, naturally occurring DNA molecules in microorganisms and bacteria, used as a medium to transfer genetic information into cells. Also, there are other ways to construct and employ plasmids containing Oligonucleotides, such as recombinant DNA and RNA sequence expression and replication and gene regulatory sequence characterization. Besides this, the insertion of Oligonucleotides is used into a gene for genome manipulation.

Furthermore, Oligonucleotides have wide use in identifying RNA and DNA sequencing as probes and in other cases, for example:

• Aptamers
• Antisense Oligonucleotides
• Molecular barcodes
• Gene expression analysis

In Conclusion

So, if you are looking for DNA or RNA Oligonucleotide synthesis services for your expression research, count on us. At Bio Basic, we offer oligonucleotide synthesizing for different research applications, including modifications, from probes to modified bases to Linkers and much more.

5 Probable Things Causing Temperature Issues with Benchtop Incubator

The term ‘Benchtop Incubator’ is quite common in the research industry, especially in the case of hematology and microbiology applications. During research applications, the experts use a benchtop centrifuge to spin samples at a high speed to separate the sample components required, for example isolating red blood cells from plasma. Then, they incubate them at a certain temperature using a benchtop incubator.

However, sometimes the incubator fails to reach the temperature you have set, preventing it from heating up or cooling down. Such interruption in between the research applications can leave you frustrated, no wonder!

Don’t worry! In today’s blog, we will discuss the factors causing such temperature issues and help you troubleshoot. Let’s get started.

Possible Reasons Impacting Benchtop Incubator Temperature

It goes without saying that it feels quite frustrating when the benchtop incubator starts malfunctioning or not working properly in the middle of a research application. But, in this case, you need to blame this host of factors mentioned below:

1: Mechanical failure-

While using an incubator shaker and you find it failing to heat or cool, don’t ignore it! It can be a serious issue, for example, a broken controller or element. In both cases, you either need to get the parts replaced or buy a new machine, such as the Incu-Shaker Mini CO2 with a nonslip rubber mat.

Now, if the benchtop incubator you use comes with a refrigeration feature but not cooling at all, the chances are of compressor failure. Similarly, here, you need to replace parts or get a new one!

2: Temperature protection-

Almost every incubated shaker comes with Over-Temperature Protection(OTP) to have a backup so it can shut off the heat if the temperature fluctuates for any reason. Hence, you can ensure stability and integrity for your samples in case of extremely high temperatures. In fact, some incubators have an automatic protection feature where the unit will turn off if the temperature goes up from the setpoint by a specific temperature, like 1°C.

But, if you set this temperature protection too low or too high, the chances are that your unit won't heat up or cool down to the temperature you desire for your research application.

3: Temperature requires stabilization-

Many incubator models have built-in reference thermometers to aid in calibration. But, if you see the temperature on the thermometer, not matching the reading of the prime temperature controller, you need to think of stabilizing the temperature. It usually happens when you have just opened the door, reset the temperature, or turned it off. The unit fails to stabilize the internal temperature due to a lack of enough time in between.

4: Broken door seal-

When the incubated shaker isn’t reaching the temperature you set, the broken door seal can be a factor to blame! After all, when the seal is damaged, the air between the outside environment and the unit replaces the warm or cold air inside and lets that out. Moreover, a broken seal can be anything, like a damaged gasket, misadjusted hinges, broken latch bolts, and much more. Look out for all these problems and repair or replace parts if possible or necessary!

5: Lack of airflow-

Benchtop incubators need sufficient free airflow to work properly. Of course, it doesn’t mean that you must have a bigger room to use it but try to keep a couple of inches gap from the walls to leave some breathing room for the unit so it can perform properly.

If your incubated shakers contain refrigeration systems, there can be ice buildup if the unit doesn’t cool sufficiently. In that case, clean the ice, and open the doors fewer to prevent moisture from entering.

In Conclusion

We hope these tips will help you troubleshoot the temperature issues with your benchtop incubator. And, if you have any of these problems gone beyond repairs or replacement, count on us to get a new one. At Bio Basic, we offer quality benchtop equipment, from incubator to fume hood to refrigerated benchtop centrifuge and Eppendorf benchtop centrifuge with high-quality performance.

So, why wait? Visit our page to find the best-suited product for your research application!