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Introduction

The 2X One-Step RT-qPCR MM (Dye) uses RNA as the template, performing RNA→cDNA→qPCR sequentially within a single reaction tube. The detection limit can reach 100pg Total RNA. This kit features high-quality reverse transcriptase and Taq DNA polymerase, along with a proprietary reaction system developed by our company, which significantly enhances amplification performance and reaction specificity. The one-step format eliminates the need for additional reagent additions or opening tubes mid-reaction, thereby preventing contamination. This technology has been optimized for dye-based real-time quantitation of target RNA sequences via Excitation (498nm), Emission (522nm) of most real-time instruments. This product supports broad instrument compatibility so no additional ROX is required for normalization.

Contents

Kit Components