Our Antibody Production

Polyclonal Antibody Production

Bio Basic's Polyclonal Antibody Service is designed to supply you with custom, affinity purified pAbs that are guaranteed to detect your antigen of choice using ELISA (titer >1:50,000) and dot blot assays. Unlike other facilities, all Bio Basic’s packages include free antigen design as well as no extra cost for additional boosts and screenings following immunization; in addition, packages which guarantee pAbs that will work in your assay of choice are also available for your ultimate peace of mind. Our peptide-supplied and protein-supplied packages are equipped to handle any antigen; our experienced scientists will conjugate your peptide and subject your protein to rigorous quality control prior to immunization to optimize serum antibody yield.

Service Protocol:

  • 1. Antigen design, synthesis and conjugation.
  • 2. Immunization of 2-4 New Zealand rabbits.
  • 3. Additional boosts and screenings (ELISA, dot blot).
  • 4. Serum separation from blood clot and serum collection (antibodies in rabbit blood).
  • 5. Affinity Purification (Protein A/G or Antigen).
  • 6. Antibody validation (ELISA, dot blot).

Description

Service Protocol

Deliverables

Timeline*

Guarantee

Price & Terms**

Standard Peptide-Antibody Package.

Required info: Name, species, sequence, and NCBI Number for target protein.

  • Peptide design, synthesis/QC and conjugation.
  • Immunization of 2 New-Zealand Rabbits.
  • Additional boosts and screenings (ELISA, dot blot)
  • Serum collection.
  • Protein A or G affinity purification.
  • Antibody validation (ELISA, dot blot).

Progress Reports.
(Includes ELISA and Dot Blot data against peptide).

Weekly.
  • Antibodies are Dot Blot positive against peptide.
  • Antibodies will have ELISA titer: > 1:50,000.

$900 + peptide synthesis

FREE Peptide Design.

FREE Additional Boosts and Multiple Screenings.

Peptide synthesis: From $288.48 for 24 mg of KLH conjugated peptide up to 16 AA length.

Shipment (1 of 1):

  • 1 mg peptide (unconjugated).
  • 20-50 ml antiserum from each rabbit.
  • All purified antibody from rabbit of choice (min. 2 mg).
  • 1-2 ml pre-immune serum from chosen rabbit.
10-12 weeks
(from antigen synthesis to final delivery).

Guaranteed Peptide-Antibody Package.

Required info: Name, species, sequence, and NCBI Number for target protein.

  • Peptide design, synthesis/QC and conjugation.
  • Immunization of 2 New-Zealand Rabbits.
  • Additional boosts and screenings (ELISA, dot blot)
  • Serum collection.
  • Antigen affinity purification.
  • Antibody validation (ELISA, dot blot).

Progress Reports.
(Includes ELISA and Dot Blot data against peptide).

Weekly.
  • Antibodies are Dot Blot positive against peptide.
  • Antibodies will have ELISA titer: > 1:50,000.
  • Antibody guaranteed to work in client’s experimental system.

$1350 + peptide synthesis

FREE Peptide Design.

FREE Additional Boosts and Multiple Screenings.

Peptide synthesis: From $288.48 for 24 mg of KLH conjugated peptide up to 16 AA length.

Shipment (1 of 2):

  • 1 mg peptide. (unconjugated).
  • 100ul antibody samples from each rabbit.
10-12 weeks
(from antigen synthesis to final delivery).

Shipment (2 of 2):

  • 20-50 ml antiserum from each rabbit.
  • All purified antibody from rabbit of choice (min. 2 mg).
  • 1-2 ml pre-immune serum from chosen rabbit.
1-2 weeks
(after confirmation of preferred antibody).

Standard Protein-Antibody Package.

Required info:
Client’s protein antigen.

  • QC client's protein (protein expression and purification if DNA is provided).
  • Immunization of 2 New-Zealand Rabbits.
  • Additional boosts and screenings (ELISA, dot blot)
  • Serum collection.
  • Protein A or G affinity purification.
  • Antibody validation (ELISA, dot blot).

Progress Reports.
(Includes ELISA and Dot Blot data against protein).

Weekly.
  • Antibodies are Dot Blot positive against protein.
  • Antibodies will have ELISA titer: > 1:50,000.

$900 + protein expression (if required)

FREE Additional Boosts and Multiple Screenings.

Additional fees will be charged for protein expression and purification if DNA is provided.

Shipment (1 of 1):

  • 1 mg protein antigen.
  • 20-50 ml antiserum from each rabbit.
  • All purified antibody from rabbit of choice (min. 2 mg).
  • 1-2 ml pre-immune serum from chosen rabbit.
10-12 weeks
(from antigen synthesis to final delivery).

Guaranteed Protein-Antibody Package.

Required info: Client’s protein antigen.

  • QC client's protein (protein expression and purification if DNA is provided).
  • Immunization of 2 New-Zealand Rabbits.
  • Additional boosts and screenings (ELISA, dot blot).
  • Serum collection.
  • Antigen affinity purification.
  • Antibody validation (ELISA, dot blot).

Progress Reports.
(Includes ELISA and Dot Blot data against peptide).

Weekly.
  • Antibodies are Dot Blot positive against protein.
  • Antibodies will have ELISA titer: >1:50,000.
  • Antibody guaranteed to work in client’s experimental system.

$1350 + protein expression (if required)

FREE Additional Boosts and Multiple Screenings.

Additional fees will be charged for protein expression and purification if DNA is provided.

Shipment (1 of 2):

  • 1 mg protein antigen.
  • 100ul antibody samples from each rabbit.
10-12 weeks
(from antigen synthesis to sample delivery).

Shipment (2 of 2):

  • 20-50 ml antiserum from each rabbit.
  • All purified antibody from rabbit of choice (min. 2 mg).
  • 1-2 ml pre-immune serum from chosen rabbit.
1-2 weeks
(after confirmation of preferred antibody).

Standard Phospho-Specific Package.

Required info:
Name, species, sequence, and NCBI Number for target protein.
Modification site information.

  • Peptide design, synthesis/QC and conjugation, (including modified peptide and unmodified control peptide).
  • Immunization of 4 New-Zealand Rabbits.
  • Additional boosts and screenings (ELISA, dot blot)
  • Serum collection.
  • Antigen affinity purification and cross-reactivity reduction.
  • Antibody validation (ELISA, dot blot).

Progress Reports.
(Includes ELISA and Dot Blot data against modified and unmodified peptides).

Weekly.
  • Antibodies are Dot Blot positive against peptide.
  • Antibodies will have ELISA titer:
    a) >1:50,000.
    b) A modified/ unmodified titer ratio >8.

$1600 + peptide synthesis

FREE Peptide Design.

FREE Additional Boosts and Multiple Screenings.

Peptide synthesis: From $695 for 24 mg of KLH conjugated peptide up to 16 AA length (synthesis of 2 peptides; one is phosphorylated).

Shipment (1 of 1):

  • 1 mg modified peptide (unconjugated).
  • 1 mg unmodified peptide (unconjugated).
  • 20-50 ml antiserum from each rabbit.
  • All purified antibody from rabbit of choice (min. 2 mg).
  • 1-2 ml pre-immune serum from chosen rabbit.
10-12 weeks
(from antigen synthesis to final delivery).

Guaranteed Phospho-Specific Package.

Required info:
Name, species, sequence, and NCBI Number for target protein.
Modification site information.

  • Peptide design, synthesis/QC and conjugation, (including modified peptide and unmodified control peptide).
  • Immunization of 4 New-Zealand Rabbits.
  • Additional boosts and screenings (ELISA, dot blot)
  • Serum collection.
  • Antigen affinity purification and cross-reactivity reduction.
  • Antibody validation (ELISA, dot blot).

Progress Reports.
(Includes ELISA and Dot Blot data against modified and unmodified peptides).

Weekly.
  • Antibodies are Dot Blot positive against peptide.
  • Antibodies will have ELISA titer:
    a) >1:50,000.
    b) A modified/ unmodified titer ratio >8.
  • Antibody guaranteed to work in client’s experimental system.

$2400 + peptide synthesis

FREE Peptide Design.

FREE Additional Boosts and Multiple Screenings.

Peptide synthesis: From $695 for 24 mg of KLH conjugated peptide up to 16 AA length (synthesis of 2 peptides; one is phosphorylated).

Shipment (1 of 2):

  • 1 mg modified peptide (unconjugated).
  • 1 mg unmodified peptide (unconjugated).
  • 100 ul sample antibodies from each rabbit.
10-12 weeks
(from antigen synthesis to sample delivery).

Shipment (2 of 2):

  • 20-50 ml antiserum from each rabbit.
  • All purified antibody from rabbit of choice (min. 2 mg).
  • 1-2 ml pre-immune serum from chosen rabbit.
1-2 weeks
(after confirmation of preferred antibody).

*: Turnaround times are estimates and can vary depending on the project.
**: For some projects, fees may apply if project has failed or is cancelled. Please inquire for full terms and details regarding guarantees.
All prices are in USD.

Project Process

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