Our Antibody Production



Monoclonal Antibody Production

Bio Basic’s monoclonal antibody service is geared towards offering custom antibodies that will simply work, regardless of the intended application.

Through collaborating with you from the beginning (antigen design and quality control), the middle (sending you supernatant samples from positive clones for evaluation) and until the end (providing your desired positive clone and purified antibody) of the entire process, we guarantee our custom monoclonal antibodies will be successful in your assay.

Service Protocol:

  • 1. 5 Balb/c mice immunised.
  • 2. B cells isolated from mice spleen.
  • 3. B cells fused with myeloma cells to form hybridomas.
  • 4. Hybridomas screened for desired antibody production (ELISA).
  • 5. Antibody-producing hybridomas cloned (2-4 rounds).
  • 6. Antibody production.
  • 7. Antibody purification (Protein A/G affinity purification).
  • 8. Antibody validation (ELISA, dot blot).

Description

Deliverables

Timeline*

Guarantee

Price & Terms**
ELISA Monoclonal Package

Progress Reports.
(Includes ELISA and Dot Blot data).

Weekly. ELISA titer: >1:100,000 $3250

Shipment (1 of 1):
a) 1 hybridoma cell line.
b) 2 mg purified antibody from 1 hybridoma cell line.
c) 50-100ul of pre-immune serum (1:10 dilution).

14-18 weeks
(from antigen synthesis to final delivery).
Guaranteed
Western Blot
Monoclonal
Package

Progress Reports.
(Includes ELISA and Dot Blot data).

Weekly.
  • ELISA titer: >1:100,000
  • Dot blot positive.
  • 1 clone works in client’s Western Blot assay.
$4800

Shipment (1 of 2):

Purified antibody samples from 3-5 hybridoma cell lines.

(Upon receipt, client tests antibody in Western Blot assay).

14-18 weeks
(from antigen synthesis to final delivery).

Shipment (2 of 2):

a) 1 hybridoma cell line.

b) 2 mg purified antibody from 1 hybridoma cell line.

c) 50-100ul of pre-immune serum (1:10 dilution).

3-4 weeks
(following confirmation of chosen antibody and hybridoma).
Guaranteed Immunohistochemistry (IHC) Monoclonal Package

Progress Reports.
(Includes ELISA and Dot Blot data).

Weekly.
  • ELISA titer: >1:100,000
  • Dot blot positive.
  • 1 clone works in client’s IHC assay.
$4800

Shipment (1 of 2):

Purified antibody samples from 3-5 hybridoma cell lines.

(Upon receipt, client tests antibody in IHC assay).

14-18 weeks
(from antigen synthesis to final delivery).

Shipment (2 of 2):

a) 1 hybridoma cell line.

b) 2 mg purified antibody from 1 hybridoma cell line.

c) 50-100ul of pre-immune serum (1:10 dilution).

3-4 weeks
(following confirmation of chosen antibody and hybridoma).
Guaranteed Immunoprecipitation (IP) Monoclonal Package

Progress Reports.
(Includes ELISA and Dot Blot data).

Weekly.
  • ELISA titer: >1:100,000
  • Dot blot positive.
  • 1 clone works in client’s IP assay.
$5780

Shipment (1 of 2):

Purified antibody samples from 3-5 hybridoma cell lines.

(Upon receipt, client tests antibody in IP assay).

14-18 weeks
(from antigen synthesis to final delivery).

Shipment (2 of 2):

a) 1 hybridoma cell line.

b) 2 mg purified antibody from 1 hybridoma cell line.

c) 50-100ul of pre-immune serum (1:10 dilution).

3-4 weeks
(following confirmation of chosen antibody and hybridoma).
Guaranteed Phospho-Specific Package

Progress Reports.
(Includes ELISA and Dot Blot data).

Weekly.
  • ELISA titer: >1:100,000
  • Dot blot positive.
  • Modified/ unmodified titer ratio >8.
  • 1 clone works in client’s assay.
$5780

Shipment (1 of 2):

a) Purified antibody samples from 3-5 hybridoma cell lines.

b) 1mg modified and 1mg unmodified peptide.

14-18 weeks
(from antigen synthesis to final delivery).

Shipment (2 of 2):

a) 1 hybridoma cell line.

b) 2 mg purified antibody from 1 hybridoma cell line.

c) 50-100ul of pre-immune serum (1:10 dilution).

3-4 weeks
(following confirmation of chosen antibody and hybridoma).

*: Turnaround times are estimates and can vary depending on the project.
**: For some projects, fees may apply if project has failed or is cancelled. Please inquire for full terms and details regarding guarantees.

Project Process

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