Next Generation Sequencing (NGS) is a fairly new technology that has expanded the capabilities of molecular biology. It goes beyond the capabilities of traditional DNA sequencing and allows the sequencing of whole genomes at a fraction of the time. Although utilized quite diverse techniques and biochemistry in each step from template library preparation, fragment amplification, to sequencing, they all adopted a massive matrix configuration popularized by microarray analysis: DNA samples on the array are simultaneously analyzed in parallel. First, massive parallelism can be achieved through ordered or disordered array configuration that offers high degree of information density. This dramatically increases the overall throughput of the sequencing operation. Second, no electrophoresis steps is required. So it's widely used in Systems Biology, Function Genomics, Epigenomics, Exon Sequencing, Transcriptome, etc.
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