The Baculovirus insect cell expression system is widely used for the production of precisely matured, folded and processed recombinant proteins. In contrast to E. coli, insect cells are able to incorporate post-translational modifications. This unique tool usually yields high amounts of the target protein, making it highly cost effective in comparison to other eukaryotic expression systems. They are also particularly well-suited for expressing secreted proteins.
|Pilot Expression: - Subcloning into recombinant bacmid DNA (baculovirus vectors). - Generation and evaluation of baculovirus recombinants (generation of high-titer viral stock). - Transfection of insect cells, SF9 cells (infection of insect cells with high titer stock). - Amplification of recombinant. - Pilot protein expression. - QC testing: SDS-PAGE and protein concentration.||- Expression results. - QC data. ‐ Weekly progress reports.||5-8 weeks||$1,600
|Pilot Purification: - Optimization of expression conditions (viral titre, incubation time, etc). - Pilot purification of expressed protein. - Max. purity currently achievable by this service is 80-90%.||
- Purified sample (variable ug quantities).
- Expression results.
‐ QC data.
- Weekly progress reports.
|Scale-up: Scale up of purified protein.||
- Desired quantity of protein.
- Expression results. - QC data. ‐ Weekly progress reports.
|Varies with scale and/or purity.||Varies with scale and/or purity.|
Expression results are provided as a PowerPoint document. Western Blot data available FREE of charge for any tagged proteins than can be detected by the anti-HIS antibody. Additional charges apply for all other proteins.
For Baculovirus Expression projects, we start with a pilot study for the expression (to test whether it is high or low), followed by purification of the pilot study if needed. The key factor here is to test the expression, as this is the main challenge for the Baculovirus expression system. You will receive a report following this study. No sample will be shipped.
Following this, you can decide the quantity and purity of protein you desire and we will provide you with a quote. The price varies based on the quantity desired and synthesis process.