To aid in the ongoing development of vaccines against COVID-19 and the Omicron B.1.1.529 variant, Bio Basic provides SARS-CoV-2 PCR Detection Kits, Viral RNA Extraction Kits, Viral Transport Medium, and ready-to-use primers and probes.
All COVID-19 related products will be made available to ship asap with quick re-supply whenever necessary. We aim to prioritize orders for all Coronavirus (COVID-19) and Omicron B.1.1.529 variant related projects for as long as it is needed.
NOTE: COVID-19 specific products are intended exclusively for basic research use in USA.
Our newly developed RT-PCR kit serves as an ideal molecular diagnostic tool to detect COVID-19/SARS-CoV-2. In order to assess the kit's performance, it has been clinically tested by a third party CRO (the analytical detection limit for the SARS-COV-2 RT-PCR Detection Kit was determined as 10 copies/reaction; Ct <37).
Highly specific for the ORF1ab and N target markers recommended by the WHO and US CDC
Ready-to-use reaction kit that includes MasterMix, Primers/Probes, and Internal Positive Control
Can be used with samples collected from sputum and naso/ oropharyngeal swabs
Compatible with standard RT-PCR machines (Applied Biosystems 7500, QuantStudio 7 Flex,
or Bio-Rad CFX96 recommended)
NOTE: SARS-CoV-2 RT-PCR Detection Kits are NOT available for sale and/or use in USA.
Cat # | Description | ||
SARS-CoV-2 RT-PCR Detection Kit (COVID-19), 50 Rxns | |||
SARS-CoV-2 RT-PCR Detection Kit (COVID-19), 500 Rxns | |||
SARS-CoV-2 RT-PCR Detection Kit (COVID-19), 1000 Rxns | |||
The Complete COVID-19 Extraction and Detection Kit (spin column) | |||
The Complete COVID-19 Extraction and Detection Kit (magnetic beads) |
*: Prices are for customers in Canada only.
These Viral RNA extraction kits are especially designed for Coronavirus (COVID-19, SARS-CoV-2) RNA Extraction. The kits simplify the isolation of viral RNA from Nasopharyngeal swabs (dry) or Viral transport medium (VTM) containing Nasopharyngeal swabs, Nasopharyngeal aspirates, and bronchoalveolar lavage (BAL) samples. The kits are available in a fast spin‐column format, or magnetic beads format where no phenol/chloroform extraction is required. Viral RNA binds specifically, while contaminants are removed in the flow‐through. PCR inhibitors such as divalent cations and proteins are completely removed, leaving pure viral RNA to be eluted in RNase‐free water. Purified RNA is ready to use in RT‐PCR detection, Northern blotting, or other downstream applications.
Kits are available in spin-column or magnetic bead formats.
Cat # | Description | |||
COVID-19 Viral RNA Extraction Kit (EZ-10 Spin Column), 50 Preps | ||||
COVID-19 Viral RNA Extraction Kit (EZ-10 Spin Column), 250 Preps | ||||
COVID-19 Viral RNA Extraction Kit (EZ-10 Spin Column), 500 Preps | ||||
COVID-19 Viral RNA Extraction Kit (MagicMag Magnetic Beads), 100 Preps | ||||
COVID-19 Viral RNA Extraction Kit (MagicMag Magnetic Beads), 1000 Preps | ||||
96‐well magnetic separation device (for Magnetic Beads) | ||||
96‐well, 2ml Deep Well Plate for magnetic separation device | ||||
micro‐centrifuge magnetic separation device (for Magnetic Beads) | ||||
Nucle@i, Nucleic acid automation machine (RNA extraction automation) |
*: Prices are for customers in Canada and USA only.
Real time RT-PCR Primer list obtained from the United States Centers for Disease Control and Prevention (CDC) - last updated March 6, 2020.
Primers, Probes and Protocol recommended by the World Health Organization (WHO) also available. Primers available include:
Name | Sequence | 2019-nCoV_N1-F | 5'-GACCCCAAAATCAGCGAAAT-3' | 2019-nCoV_N1-R | 5'-TCTGGTTACTGCCAGTTGAATCTG-3' | 2019-nCoV_N1-P | 5'-FAM-ACCCCGCATTACGTTTGGTGGACC-DQ*-3' | 2019-nCoV_N2-F | 5'-TTA CAAACATTGGCCGCAAA-3' | 2019-nCoV_N2-R | 5'-GCGCGACATTCCGAAGAA-3' | 2019-nCoV_N2-P | 5'-FAM-ACAATTTGCCCCCAGCGCTTCAG-DQ*-3' | 2019-nCoV_N3-F | 5'-GGGAGCCTTGAATACACCAAAA-3' | 2019-nCoV_N3-R | 5'-TGTAGCACGATTGCAGCATTG-3' | 2019-nCoV_N3-P | 5'-FAM-AYCACATTGGCACCCGCAATCCTG-DQ*-3' | RNAse P (RP-F) | 5'-AGATTTGGACCTGCGAGCG-3' | RNAse P (RP-R) | 5'-GAGCGGCTGTCTCCACAAGT-3' | RNAse P (RP-P) | 5'-FAM-TTCTGACCTGAAGGCTCTGCGCG-DQ*-3' | RdRP_SARSr-F2 | 5'-GTGARATGGTCATGTGTGGCGG-3' | RdRP_SARSr-R1 | 5'-CARATGTTAAASACACTATTAGCATA-3' | RdRP_SARSr-P2 | 5'-FAM-CAGGTGGAACCTCATCAGGAGATGC-DQ*-3' | RdRP_SARSr-P1 | 5'-CCAGGTGGWACRTCATCMGGTGATGC-DQ*-3' | E_Sarbeco_F1 | 5'-ACAGGTACGTTAATAGTTAATAGCGT-3' | E_Sarbeco_R2 | 5'-ATATTGCAGCAGTACGCACACA-3' | E_Sarbeco_P1 | 5'-ACACTAGCCATCCTTACTGCGCTTCG-DQ*-3' |
*: Where DQ is a Dark Quencher which is equivalent in function to the suggested Dark Quencher by the CDC.
**: Prices are for customers in Canada and USA only.
More primers and probes available than listed, please inquire with our oligo synthesis team at synthesis@biobasic.com.
Viral Transport Medium (VTM) is used to collect and transport viruses in an active or inactive form to the laboratory for isolation. It is designed to maintain the viability and the virulence of the viral sample (or to keep the virus in an inactive state with it's RNA preserved) while it is transported to the laboratory.
Cat # | Description | |||
Active Transfer Medium, HANKS Buffer Based | ||||
Inactive Transfer Medium, Preservative Buffer |
*: Prices are for customers in Canada and USA only.
Guanidine Thiocyanate (Guanidinium thiocyanate or guanidinium isothiocyanate), a powerful protein denaturant, is most often used to inactivate endogenous RNases in the isolation of RNA from various tissues and bacteria. When used with acid-equilibrated phenol or phenol:chloroform, a solution of Guanidine Thiocyanate and beta-mercaptoethanol are very effective in disrupting both cytoplasmic and nuclear membranes while maintaining the integrity of the RNA. In addition, Guanidine Thiocyanate is also used as a storage buffer for blood samples.
Cat # | Description | ||||
Guanidine thiocyanate (GITC), DNase, RNase, Protease Free | 593-84-0 | ||||
Guanidine thiocyanate (GITC) | 593-84-0 |
*: Prices are for customers in Canada and USA only.
Our polypropylene tubes are chemical inert withstand centrifugation speed of 3000RPM.
Cat # | Description | ||||
10ml Tube and Screw Cap, Natural, Polypropylene, Sterile, 100/Bag | |||||
10ml Tube and Screw Cap, Red, Polypropylene, Sterile, 100/Bag | |||||
10ml Tube and Screw Cap, Blue, Polypropylene, Sterile, 100/Bag | |||||
10ml Tube and Screw Cap, Yellow, Polypropylene, Sterile, 100/Bag | |||||
10ml Tube and Screw Cap, Natural, Polypropylene, 100/Bag |
*: Prices are for customers in Canada and USA only.
Bio Basic offers swabs for the collection of nasal and oral samples.
Cat # | Description | |||
Specimen Collection Swab, Sterile, Nasal | ||||
Specimen Collection Swab, Sterile, Oral |
*: Prices are for customers in Canada and USA only.
RNA columns can be used to purify both single- or doublestranded DNA or RNA from contaminants such as salts, solvents, enzymes or proteins.
Cat # | Description | |||
EZ-10 RNA Column and collection tube (clear tube, clear ring, clear collection) |
*: Prices are for customers in Canada and USA only.
Bio Basic's dNTP mixture is a routine reagent for use in a variety of PCR based applications.
DNase, RNase: None detected.
Suitable for use in the Polymerase Chain Reaction (PCR).
PCR Suitability
Cat # | Description | |||
dNTP mixture (10mM) | ||||
dNTP mixture (25mM) |
*: Prices are for customers in Canada and USA only.
Proteinase K is used to digest protein and remove contamination from preparations of nucleic acid. Proteinase K rapidly inactivates nucleases that may otherwise degrade the RNA during purification.
Cat # | Description | |||
Proteinase K | ||||
Proteinase K | ||||
Proteinase K |
*: Prices are for customers in Canada and USA only.
Bio Basic offers Gene Servicesfor projects related to the COVID-19 and the Omicron variant.
Please mention "Coronavirus", "COVID-19", "Omicron", "booster" or "vaccine" in the comments section upon checkout.