Below are some frequently asked questions about our Gene Synthesis services. Should you have any questions other than listed below, feel free to contact us at gene.request@biobasic.com and we will be happy to clear your thoughts.
Generally, the deliverables for your synthetic gene of interest will consist of the following:
2-4ug of lyophilized DNA.
Sequence chromatogram tracefiles.
Sequencing Results.
Final plasmid sequence.
Alignment files.
Reconstitution, re-plating and bacteria growth instructions.
Vector information.
Gene report (includes restriction digestion data).
Please note that we keep stock of final plasmid deliverables for 3 months, unless otherwise requested.
We have a simple Gene Project Tracker portal where you can use your designated BBI ID(s) to track your project progress.
Bio Basic standard cloning vectors include pUC57-Amp, pUC57-Kan, pUC-SP and pBluescript . In general, synthetic genes will be cloned via Blunt of the standard vector, or any cloning sites you desired. Click here for a list and details on our cloning vectors.
Yes, Bio Basic can subclone the synthetic gene into the vector of your choice at an affordable additional cost. The vector (commercial vector or non-commercial vector) must be provided to us with a full-length sequence and other requested info.
Bio Basic accepts credit cards (Visa, MasterCard, American Express), cheques and wire transfer. You may send us a PO if you are an academic institution. For certain orders, Bio Basic may require a deposit.
Yes! Bio Basic provides protein expression and purification services using bacteria, yeast, baculovirus, and mammalian cell systems. For more information, please visit our Protein Production service page.
Yes. The sequences of genes synthesized by us are confirmed by DNA sequencing, and 100% guaranteed.
Generally, it is more difficult to synthesize sequences that have:
Please click here for the terms and conditions for our gene synthesis related services.
Please click here for instructions on how to use the portal.
Please send two tubes each containing 4.0 ug of plasmid DNA to:
Bio Basic Inc.
ATTN: Gene Synthesis Department
20 Konrad Crescent
Markham, ON
L3R 8T4
Please include a memo with your project number so we can easily identify the package.
Please contact us at gene.request@biobasic.com if you have any questions or concerns regarding shipping.
All our genes undergo column-based purification and as a result, may be suitable for transfection.
If further purification is needed to ensure near-complete removeal of endotoxin, please request our endotxin-free prep service.
Up to 6 months at room temperature, 1 year if refrigerated (2 to 8°C) and up to 2 years if frozen (-20 to -30°C).