Escherichia coli (E. coli) is one of the most widely used hosts for the production of heterologous proteins. Its genetics are far better characterized than those of any other microorganism.
E.coli expression is by far the most cost-efficient and consistent production method. We have the capabilities to scale up from milligrams to grams. Although E.coli do not incorporate post-translational modifications into the recombinant protein, many E.coli produced proteins do show activity of the native protein (our robust catalogue of active recombinant proteins is evidence of this). However bacterial cells produce endotoxins, so if you plan to use the protein in any animal cells or assays, endotoxin removal is recommended.
Description | Deliverables | Turnaround | Price |
---|---|---|---|
‐ 0.5 to 1L Expression and purification ‐ Guaranteed 1 mg of purified protein ‐ QC Testing (SDS-PAGE and Western Blot) |
‐ Weekly progress reports ‐ 1 mg of purified protein ‐ QC data |
2‐3 weeks | $1667.50 USD |
‐ 1L Expression and purification ‐ Guaranteed 3 mg of purified protein ‐ QC Testing (SDS-PAGE and Western Blot) |
‐ Weekly progress reports ‐ 3 mg of purified protein ‐ QC data |
2‐3 weeks | $2183.85 USD |
‐ 1 to 2L Expression and purification ‐ Guaranteed 5 mg of purified protein ‐ QC Testing (SDS-PAGE and Western Blot) |
‐ Weekly progress reports ‐ 5 mg of purified protein ‐ QC data |
2‐3 weeks | $2426.50 USD |
‐ 2 to 3L Expression and purification ‐ Guaranteed 15 mg of purified protein ‐ QC Testing (SDS-PAGE and Western Blot) |
‐ Weekly progress reports ‐ 15 mg of purified protein ‐ QC data |
2‐6 weeks | $3312.00 USD |
NOTE:
1. Pricing applies only if gene construct is provided.
Gene synthesis services are available for an
additional cost.
2. The size of the target protein must be less than
100 kDA.
3. In rare cases, we may use alternative tags to
purify a protein. The final protein may be tag
containing.
4. Pricing does not include tag removal or endotoxin removal.
Additional charges apply for these
services.
5. We guarantee protein solubility but DO NOT
guarantee the bioactivity or functionality of the
protein. If the protein expresses as inclusion bodies,
refolding will be done to make the protein soluble.
6. Quantity guaranteed or it is FREE.
7. Western Blot data is available FREE of charge for
any tagged proteins than can be detected by the
anti-HIS antibody. Additional charges apply for all
other proteins.
Description | Deliverables | Turnaround | Price |
---|---|---|---|
‐ Provide your expression constructs, or we’ll sub-clone your gene into our expression vector. ‐ Expression pilot study: expression optimization and verification. ‐ Purification Options: A. We follow your published or unpublished purification protocol. B. Let us optimize conditions and choose the best course of action for purification of your protein (no extra charge). ‐ Large scale expression and purification at your desired quantity (mg to g). ‐ Tag removal, refolding, endotoxin removal, and other steps as needed according to your requirements. ‐ QC testing: SDS‐PAGE and protein concentration (Western Blot) |
‐ Weekly progress reports ‐ Desired quantity of tag removed, purified, soluble protein ‐ QC data |
Varies (Please inquire) |
From $1600 (Please inquire) |
PROJECT PROCESS:
Description | Deliverables | Turnaround | Price |
---|---|---|---|
- Sub-cloning into expression vector - Expression pilot study: expression optimization and verification - 1 mg purified protein, native form - Large scale expression (2 to 3L) and purification - Tag removal - QC testing: SDS-PAGE and protein concentration (Western Blot) |
- Weekly progress reports - 1 mg of tag removed, purified, - Protein in its native form - 90 to 95% purity - Endotoxin level <1eu/ug - QC Data |
4-6 weeks | $2928.90 USD |
- Sub-cloning into expression vector - Expression pilot study: expression optimization and verification - 5 mg purified protein, native form - Large scale expression (5 to 10L) and purification - Tag removal - QC testing: SDS‐PAGE and protein concentration (Western Blot) |
- Weekly progress reports - 5 mg of tag removed, purified, - Protein in its native form - 90 to 95% purity - Endotoxin level <1eu/ug - QC Data |
4-8 weeks | $7040.00 USD |
NOTE:
Western Blot data available FREE of charge for any tagged
proteins than can be detected by the anti-HIS antibody.
Additional charges apply for all other proteins.