PCR Products

1. Dissolve final PCR product in ddH2O (DO NOT use TE Buffer*).
2. Crude PCR products concentration:
>30ng/ul, Volume: >50ul.**
Purified PCR products concentration:
>30ng/ul, Volume: >20ul.**
For PCR products >2kb, more DNA may be required.
3. Please indicate the length of insert (bp).
4. A single distinct band of final PCR product should be detected on gel. If smearing or multiple bands are seen, you may receive poor or no readable sequencing data.
5. To avoid cross contaminations during shipment, please use a tight-fit lid for samples in 96-well format. Samples can also be shipped in 8-strip PCR tubes.

* = TE buffer contains both Tris and EDTA. The presence of EDTA in buffer will chelate the Mg2+ ion (a required cofactor for polymerase), thereby making it inaccessible to the polymerase for the sequencing reaction.

** = Volume specified is sufficient for ~ 3 reactions.
We prefer larger sample volumes to minimize delays if repeat sequencing, or troubleshooting is required.
Alternatively:
Minimum required volume for crude PCR samples: 15ul
Minimum required volume for purified PCR samples: 7ul