Plasmids

1. Dissolve purified plasmid DNA in 30~50ul ddH2O (DO NOT use TE Buffer*).
2. Concentration of plasmid should be >100-200ng/ul.
3. It is recommended to use a molecular biology kit to purify plasmid DNA rather than ethanol precipitation.
4. To avoid cross contaminations during shipment, please use a tight-fit lid for samples in 96-well format. Samples can also be shipped in 8-strip PCR tubes.

* = TE buffer contains both Tris and EDTA. The presence of EDTA in buffer will chelate the Mg2+ ion (a required cofactor for polymerase), thereby making it inaccessible to the polymerase for the sequencing reaction.