Gene Synthesis Applications: Explore in Brief

Admit it or not, making genes may have never been so easy until the emergence of gene synthesis technology. During traditional methods, the selected genes are extracted, amplified and put for sequencing through different molecular assessments. For genetic research, this method is crucial, but the overall technique of gene-extracting sequences and mutation is quite laborious.

That’s why here is where gene synthesis comes in. It’s a groundbreaking technique introducing the same interest alteration. This synthetic biology method has wide applications in today’s world.

And at Bio Basic, we offer gene synthesis services with an over 95% completion rate, even on the most challenging sequences. But before you outsource our services, have a brief idea of its applications in different fields. Let's get started:

Gene Synthesis in Molecular Biology

Recent studies show that clarified amino acid and nucleotide sequences involve protein and polypeptide genes for actual research and production. So far, the expression of different gene synthesis has become successful in molecular biologies, like interferon, human growth hormone, insulin, interleukin etc.

Gene Synthesis in Synthetic Biology

Synthetic biology refers to metabolic engineering (interfacing natural cellular metabolic methods). In this gene synthesis application technique, the specialists program cells for different practical applications. For example, it can involve affordable drugs or chemical synthesis or a biosynthetic artemisinin pathway construction in yeast to enable microbial production for such antimalarial compounds. And the best part is that this gene synthesis service costs one-tenth of the traditional harvesting production technique from the Artemisia annua plant.

Application of Gene synthesis in medicines

With the advancement of technology, it becomes quite sophisticated and easier to understand diseases, whether it be at the tissue or organ level or molecular or genetic level. Thus, tackling genetic diseases may have never been so simple before this new discovery. For instance, fluorescent probes are a valuable tool in the field of clinical diagnosis, detecting harmful deletions in different genes.

Now, let’s talk about the gene synthesis process we follow at Bio Basic. Such as:

Oligonucleotide synthesis-

In this first step, our gene synthesis specialists chemically synthesised long primers of ~50-60 bases in length at a similar melting temperature as fragments) for the desired gene sequence. We design primers to assemble each one during overlapping sequences.

PCR amplification-

Our experts assemble the primer fragments using PCR into multiple blocks to double-stranded DNA up to 1kb. Then, we assemble and amplify the blocks again similarly and construct a large double-stranded DNA.

Fragment sequencing-

We verify all the genes using DNA sequencing and select only the correct sequences. In this phase, our gene synthesis specialists use Sanger sequencing for its short run time and long read length.

Identifying Fragment errors-

During this event, our specialists perform fragment error correction to rectify mutated bases. It ensures that the gene synthesis process is completed as per the specifications desired for the project.

Vector Insertion and clone selection-

In this step, we insert the synthesised DNA into a certain amplified and isolated vector. As a default vector, we use the pUC57-Amp vector. After that, we select the appropriate and grown clones containing synthetic genes.

Gene Sequencing: First Round-

This process is the same as fragment sequencing we have discussed above using Sanger Sequencing. Then, we correct the errors as planned for the project.

Gene Sequencing: Second Round-

Similarly, the final round of gene sequencing goes on, and after that, our specialists apply further QC measures and lyophilize the gene into the microcentrifuge tube for downstream applications.

Bottom Line

So if you want gene synthesis services from us, Bio Basic, just go to the Gene Order Portal to get an automated quote and submit your gene order now! Click here to know how to use the portal!