Our Oligo Purification Types

HAP Purified Oligos

Bio Basic’s proprietary method of High Affinity Purification (HAP).

Oligos significantly more pure than the Desalted method.

Salts removed.

Some failure sequences removed.

Purity: >75%*.

Length: 11-59 bases.

Application: PCR, Sequencing.

Final Yield and Pricing

Final Yield (based on oligo length) Pricing
5-59 bases 11-59 bases 60-99 bases 100-130 bases per base ($) per oligo ($)**
- 2 OD - - $0.19 -
- 5 OD - - $0.40 -
- 10 OD - - $0.45 -

* = Based on an oligo length of 30 bases.

** = An additional purification cost per oligo for HPLC (non-modified) and HPLC-CE purified oligos.

Remember that the synthesis scale (25 nmol, 100 nmol etc.), refers to the STARTING MATERIAL for oligo synthesis, and NOT the FINAL yield. Oligo length, sequence, GC content and coupling efficiencies alter the final nmol yield. Different purification methods alter the final yield as well. Bio Basic guarantees the final yield of each oligo nucleotide as a fixed yield in OD units.

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