High Performance Liquid Chromatography (HPLC) based
Recommended for shorter oligos (5-59 bases)
Salts removed
Majority of failure sequences removed
MS data provided
Purity: >85%*
Oligo length: 5-130 bases
Application: Cloning, Gel Shift Assay, Mutagenesis
Final Yield (based on oligo length) | Pricing | ||||
---|---|---|---|---|---|
5-59 bases | 11-59 bases | 60-99 bases | 100-130 bases | per base (USD) | per oligo (USD)** |
2 OD | - | - | - | $0.20 | $6.00 |
5 OD | - | 2 OD | - | $0.35 | $10.00 |
10 OD | - | 5 OD | 2 OD | $0.55 | $14.00 |
* = Based on an oligo length of 30 bases.
** = An additional purification cost per oligo for HPLC (non-modified) and HPLC-CE purified oligos.
Remember that the synthesis scale (25 nmol, 100 nmol etc.), refers to the STARTING MATERIAL for oligo synthesis, and NOT the FINAL yield. Oligo length, sequence, GC content and coupling efficiencies alter the final nmol yield. Different purification methods alter the final yield as well. Bio Basic guarantees the final yield of each oligo nucleotide as a fixed yield in OD units.